PRECLINICAL DMPK APPLICATIONS
NORMOTHERMIC PERFUSED PORCINE ORGANS
CONTRACT SERVICE, TECHNOLOGIES OPEN FOR PARTNERING
Ex vivo whole liver normothermic machine perfusion model

Prediction of First Pass Liver Effects, Biliary Excretion and DDI
● Porcine Liver model (for contract research)
> Hepatic Clearance
> Hepatic Metabolism
> Biliary Excretion
> Plasma Exposure upon DDI
> Dosing to portal vein and/or hepatic artery
● Human Liver model (for collaboration research)
> Hepatic Clearance
> Hepatic Metabolism
> Biliary Excretion
> Plasma Exposure upon DDI
> Dosing to portal vein and/or hepatic artery
> NASH: Biomarker detection / Target Binding
Download:
●
Normothermic Machine Perfusion of Diseased Explanted Livers to Study Hepatic Pharmacokinetic Processes (Poster at ISSX-2021)
●
Ex vivo whole liver perfusion model for prediction of drug drug interactions and biliary excretion of Rosuvastatin (Poster)
●
Pharmacokinetics Application of Normothermic Perfused Ex Vivo Porcine Livers. Poster ISSX-2019
Ex vivo Kidney perfusion model
TO STUDY RENAL CLEARANCE OF DRUGS
● Experimental set up;
> Infusion of drug cocktail to study renal clearance of probe drug cocktail (0 - 120 min)
> Study DDI at the renal level by infusing probe + inhibitor cocktail mix (125- 245 min)
> Perfusate and urine drug profiles
● Measurement;
> Flow (pressure driven machine)
> Inulin clearance
> Urine production
> Ad hoc viability analysis
> Perfusate: pO2, pCO2, pH, sO2, ureum,creatinin, Na, K
> Albumin (in perfusate and urine)
> Biopsy: before, during & end of perfusion
Combined Liver and Kidney perfusion model
To study Clearance of Probe Drug Cocktail via Biliary and Renal Clearance route
● Determine % of biliary and % renal excretion of drugs
● Ability to perfuse for longer time
● Open for Partnering